I. Definition Radioimmunoassay (RIA) is a very sensitive medical technique used to measure the concentrations of antigens, such as the quantification of the binding or inhibition of binding of a radiolabeled antibody to a substance that helps the physician to determine that the sterility is not or caused by a hormonal imbalance during the menstrual cycle.
II. Procedure By mixing a known amount of an antigen and the antibody with the serum sample from a patient containing an unknown amount, can measure the interaction of testosterone, LH, FSH, prolactin hormones.
III. Diagnosis After the lining of the uterus is shed and the end of the previous menstrual cycle. Increased estrogen indicate the menstrual cycle begins again. Estrogen levels stimulate the hypothalamus of the secret gonadotropin releasing hormone (GnRH), which helps the pituitary gland secretes follicle stimulating hormone (FSH) for the production of eggs in the ovaries. When the eggs mature, the production of luteinizing hormone (LH) causes mature egg from the ovaries is extruded few days before ovulation and allow the cervix to produce mucus sperm friendly then the increase in progesterone levels to soften the wall of the uterus for implantation of the fertilized embryo if otherwise the lining of the uterus is shed and prepare the next menstrual cycle. Everything that went wrong in the secretion of the hormone during this process influences the quantification of binding or binding inhibition which can be detected by radiolabeled substance to an antibody.
IV. Risk There are no known risk.
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